Ken, I believe, according to CuteChat install instructions for DotNetNuke is to add the following to the Global.asax file (root web directory).  Here are the contents of our file:

 

<%@ Application Inherits="DotNetNuke.Common.Global" Language="VB" %>
<script Runat=server>
Public Overrides Sub Init()
    MyBase.Init()
    If Not CuteChat.ChatSystem.HasStarted Then
        CuteChat.ChatProvider.Instance = New DNNChatProvider()
        CuteChat.ChatSystem.Start(New CuteChat.AppSystem())
    End If
End Sub
</script>